L02 细胞建立人鼠嵌合肝动物模型
http://www.100md.com
林 沪, 毛 青, 王宇明, 蒋业贵, 邓国宏,
嵌合肝;L02肝细胞;动物模型;大鼠,L02细胞建立人鼠嵌合肝动物模型,林沪,毛青,王宇明,蒋业贵,邓国宏,李俊刚,刘国栋,林沪,通讯作者:,Establishmentofananimalmode
 |
| 第5页 |
 |
| 第1页 |
参见附件(559KB，6页)。
林沪, 毛青, 王宇明, 蒋业贵, 邓国宏, 李俊刚, 刘国栋, 中国人民解放军第三军医大学西南医院感染病研究所 重庆市 400038
林沪, 男, 1970-01-08, 重庆市人, 汉族, 2003年第三军医大学西南医院感染病研究所硕士, 主治医师, 主要从事肝炎发病机制和病毒性肝炎动物模型的研究.
国家自然科基金资助项目, No.30271173
通讯作者: 林沪, 400038, 重庆市沙坪坝区高滩岩正街30号, 中国人民解放军第三军医大学西南医院感染病研究所. linhu7018@yahoo.com.cn
电话: 023-65399064 传真: 023-68754475
收稿日期: 2005-10-25 接受日期: 2005-10-31
Establishment of an animal model of tolerant rats with chimeric human livers by human L02 hepatocytes
Hu Lin, Qing Mao, Yu-Ming Wang, Ye-Gui Jiang, Guo-Hong Deng, Jun-Gang Li, Guo-Dong Liu
Hu Lin, Qing Mao, Yu-Ming Wang, Ye-Gui Jiang, Guo-Hong Deng, Jun-Gang Li, Guo-Dong Liu, Institute of Infectious Disease, Southwest Hospital of the Third Military Medical University, Chongqing 400038, China
Supported by National Natural Science Foundation of China, No.30271173
Correspondence to: Hu Lin, Institution of Infectious Disease, Southwest Hospital of the Third Military Medical University, Chongqing 400038, China. linhu7018@yahoo.com.cn
Received: 2005-10-25 Accepted: 2005-10-31
Abstract
AIM: To investigate whether human L02 hepa-tocytes can survive after implantation to the normal, immunocompetent rats.
METHODS: Human L02 hepatocytes were injected into the peritoneal cavities of fetal Sprague Dawley rats to induce immune tolerance to human L02 hepatocytes. After DiI staining, the hepatocytes were implanted to the 2-week rats via the spleen. Immuno-fluorescent staining, SP immunohistochemistry, and DiI staining were used to detect human albumin and specific proliferating cell nuclear antigen (PCNA) in the rat liver. The distribution of human L02 hepatocytes was observed under fluorescent microscope.
RESULTS: Dynamic distribution of human L02 hepatocytes in the rat livers was observed from 1st to 10th week after implantation. Human albumin was detected at 2, 4, 6, and 8 wk, and the most content appeared at 4 wk ......
您现在查看是摘要介绍页，详见PDF附件(559KB，6页)。