关键词: 血管抑素;真核表达;载体;转染

    中图号:Q791

    摘 要:目的 将血管抑素基因转染入人肝癌细胞SMMC-7721中，观察其表达效果及其对细胞本身的影响，并探讨血管抑素治疗动物种植性肿瘤的效果. 方法 将鼠源性血管抑素基因插入真核表达载体pcDNA3.1(+)，构建重组质粒，用HindⅢ和XbaⅠ双酶切及基因测序进行鉴定.然后用脂质体法将重组质粒pcDNA3.1-mAST转染入人肝癌细胞SMMC-7721中，并行RT-PCR鉴定和Western-blot检测其表达效果. 结果 鼠血管抑素基因成功克隆入真核表达载体pcD-NA3.1(+)中;经脂质体法转染入SMMC-7721细胞并获稳定表达;转染血管抑素和未转染血管抑素的SMMC-7721细胞生长速度无明显差异. 结论 鼠血管抑素基因在人肝癌细胞SMMC-7721中可获稳定表达，但对细胞本身生长无明显抑制作用.

    Stable expression and significance of murine angio┐statin cDNA on human hepatocellular cancer cell line SMMC┐7721

    TAO Kai-Shan，WU Xing-An，DOU Ke-Feng

    Department of Hepatobiliary Surgery，Xijing Hos-pital，Department of Microbiology，Faculty of Pre-clinical Medicine，Fourth Military Medical Universi-ty，Xi'an710033，China

    Keywords:angiostatin;eukaryotic expression;vector;trans-fection

    Abstract:AIM To prepare for the investigation of the an-giostatin cDNA treatment on primary tumor，the angiostatin cDNA was transfected into the human hepatocellular cancer cell line SMMC-7721and the expression results and it's effect on cells were observed.METHODS The murine angiostatin cDNA was transfected into eukaryotic expression vector of pcDNA3.1(+)to construct the restructing plasmid ......