关键词: 高血压;妊娠并发症;胎盘;基因克隆;PCR;减法杂交

    中图号:R714.246

    摘 要:目的 应用妊娠高血压综合征(妊高征)患者胎盘研究其发病相关基因. 方法 利用基于PCR的改良消减杂交技术，首先建立了该病相关的cDNA消减文库.随机筛选出20个含有差异表达片段的克隆，经过反向点杂交，弃掉6个假阳性克隆，确定14个克隆为妊高征发病相关基因. 结果 经过序列测定和同源性比较(BLAST)，发现11个为已知基因，其中1号基因为necdin家族基因;3个为新基因.这3个新基因已完成了全部插入片段的序列测定，并登录GenBank，登录号为AF232216，AF232217，AF233648. 结论 妊高征患者胎盘基因表达谱和正常妊娠胎盘相比，发生改变.这可能与妊高征的发病有关.

    Cloning of genes related to pregnancy induced hy┐pertension syndrome

    SHI Juan-Zi，YAO Yuan-Qing，YAN Wei，ZHU Feng，ZHAO Zhong-Liang，LIU Yan-Fang

    Department of Pathology，Department of Bio-chemistry and Molecular Biology，Faculty of Pre-clinical Medicine，Fourth Military Medical Universi-ty，Xi'an710033，China，Department of Obstetrics and Gynaecology，Tangdu Hospital，Fourth Mili-tary Medical University

    Keywords:hypertension;pregnancy complication;placenta;geneclone;PCR;subtractive hybridization

    Abstract:AIM To clone genes specifically expressed in pla-centa of pregnancy induced hypertension syndrome(PIHs)，and to explain their significance in the etiopathology of PIHs.METHODS The placentas of PIHs and normotensive were used as modals ......