关键词: 电针;足三里;胃泌素;受体，表皮生长因子;胃粘膜;免疫组织化学

    摘 要:目的 观察电针足三里穴对胃粘膜细胞受体表达及核增殖的影响，探讨针灸作用机制. 方法 75只SD大鼠随机分段分为5组，在清醒状态下针刺实验，SABC法免疫组化染色观察胃粘膜细胞胃泌素(Gas)、表皮生长因子受体(EGFR)表达程度，AgNOR特染观察胃腺细胞增殖情况. 结果 电针足三里穴胃粘膜EGFR阳性表达率73.3%，Gas表达率66.7%;电针三阴交对EGFR表达无影响，Gas表达率为46.7%;联合穴位组Gas，EGFR表达均达60.0%，AgNOR特染各组核内嗜银颗粒无明显差异. 结论 电针足三里可提高胃粘膜细胞Gas和EGFR表达率，改变细胞分子结构，影响细胞功能.电针对胃粘膜细胞核增殖无影响.

    Zusanli electroacupuncture enhances expression of Gas and EGFR in gastric mucosa in rats

    ZHAO Bao-Min，HUANG Yu-Xin，ZHANG Wei，ZHAO Ning-Xia，LIU Jie，WANG Qing-Li ，WANG Lian-Gang

    1 Department of Gastroenterology，

    2 Department of Pathology，

    3 Department of Traditional Chinese Medicine，Tangdu Hospital，Fourth Military Medi-cal University，Xi'an710033，China，

    4 Department of Experimental Surgery，Xijing Hospital，Fourth Millitary Medical University

    Keywords:electroacupuncture;zusanli;gastrin;epidermal growth factor receptor;gastric mucosa;im-munocytochemistry

    Abstract:AIM To observe the effect of electroacupuncture stimulation of zusanli on the expression of Gas and EGFR in gastric mucosa in order to probe mechanisms of acupuncture.METHODS 75SD rats were randomized into5groups:Control group ......