抗角蛋白自身抗体亲和力成熟及其免疫学意义
兔,,兔;自身抗体;角蛋白;抗体亲和力,1材料和方法,2结果,3讨论,参考文献
关键词: 兔;自身抗体;角蛋白;抗体亲和力中图号:R446.62;R758.2
摘 要:目的 检测同种抗角蛋白自身抗体(rAK auto Ab)反复注射家兔后,其血清AK auto Ab与角蛋白亲和力的动态变化. 方法 采用亲和层析法纯化rAK auto Ab,5mg kg-1 肌肉注射,隔日一次,连续8wk,ELISA法检测不同时间点家兔血清中AK auto Ab与家兔角蛋白的反应强度. 结果 实验组家兔血清A490nm 随时间推移而增加,对照组家兔血清A490nm 则基本保持不变. 结论 长期大量应用rAK auto Ab的家兔血清中,rAK auto Ab与角蛋白的亲和力随时间推移而增加.
Affinity maturation of antikeratin autoantibody and its immunological significance
XIA Ru-Shan,LIU Yu-Feng,LI Cheng-Xin,LI Wei
Center of Dermatology&Venereology of Chinese PLA,Xijing Hospital,Fourth Military Medical U-niversity,Xi'an710033,China
Keywords:rabbits;autoantibodies;keratin;antibody;affini-ty
Abstract:AIM To observe the dynamic changing of the affinity of sera antikeratin autoantibodies to keratin after allo-genic AK auto Ab was injected with large dose for long-term.METHODS Allogenic AK auto Ab was purified by affinity chromagraphy colume and was injected(5mg/kg)intramus-cularly to rabbit once every other day for8weeks.Affinity of sera antikeratrin autoantibodies to keratin was detected by ELISA.RESULTS A gradually ......
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