关键词: 神经营养素;背根神经节;腺病毒;基因治疗

    摘 要:目的 观察神经营养素-3(neurotrophin3，NT-3)重组腺病毒(adenovirus vector for NT-3，Ad-NT-3)对脊髓背根神经节(dorsal root ganglia，DRG)细胞存活的促进作用. 方法 原代培养DRG，加入不同感染增殖率(multiplicity of infection，MOI)的Ad-NT-3，观察DRG细胞的形态，MTT法测定DRG细胞的增殖活性. 结果 当加入MOI为10和50的Ad-NT-3时，形态学观察发现，DRG细胞数目增加，突起变长，尤其以3d后表现更为明显;DRG细胞的增殖活性显著增加(P<0.01).当MOI为100时1d和3d，DRG细胞数目明显减少，无突起或变短，部分细胞死亡;MTT法测定DRG细胞的增殖活性均显著降低(P<0.01). 结论 NT-3基因能通过腺病毒介导表达NT-3蛋白，促进体外培养DRG细胞的存活.

    Promoting survival of dorsal root ganglia by aden┐oviral┐mediated neurotrophin┐3expression in vitro

    ZHU Jin-Yu，ZHU Qing-Sheng，HUANG Yao-Tian，YANG Hao，LI Shun-Li

    1 Institute of Orthopaedics of Chinese PLA，Xijing Hospital，

    2 Institute of Neurosciences，Fourth Mili-tary Medical University，Xi'an710033，China，

    3 Department of Orthopaedics，461Hospital of Chi-nese PLA，Changchun130021，China

    Keywords:neurotrophin;dorsal root ganglia;adenoviridae;gene therapy

    Abstract:AIM To investigate the effect of promoting sur-vival of adenovirus vector for neurotrophin3(NT-3)(Ad-NT-3)on dorsal root ganglia(DRG).METHODS Primary culture of DRG cells was established.To the experimental groups Ad-NT-3were added in different multiplicity of infec-tion(MOI)respectively.Groups added with culture medium served as control.The morphology and MTT test of the DRG cells were detected.RESULTS When the MOI was between10and50 ......