高效液相色谱测定法测定盐酸精氨酸葡萄糖注射液中盐酸精氨酸的含量
高效液相色谱测定法,,高效液相色谱测定法;盐酸精氨酸;含量,1仪器与试剂,2方法与结果,3讨论
【摘要】 目的 研究建立高效液相色谱测定法测定盐酸精氨酸葡萄糖注射液中盐酸精氨酸的含量。方法 以氨基键合硅胶为填充剂,流动相:乙腈-磷酸盐缓冲液(50:50);检测波长:200nm;流速:1.0ml/min。结果 盐酸精氨酸在0.025~0.5mg/ml范围内与峰面积呈良好线性关系,r=0.9998,平均回收率为99.64%(n=9,RSD=0.97%)。本方法简便、快速、准确、重现性好,可用于本品的质量控制。【关键词】 高效液相色谱测定法;盐酸精氨酸;含量
【Abstract】 Objective To establish a HPLC method for determination of the content of arginine hydrochloride in arginine hydrochloride and glucose injection.Methods C-NH2 column;The mobile phase was acetonitrile-water(50:50);The detection wavelength was 200nm.The flow rate was 1.0ml/ml;The colum temperature was 40℃.Results There was good linearity of arginine hydrochloride in the range of 0.025~0.5mg (r=0.9998 n=6).the average recovery was 99.64% (n=9 ......
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