关键词: 胃肿瘤;血管内皮生长因子;反义核酸 中图号:R730.23 文献标识码:A

    摘 要:目的 利用本室构建的反义VEGF165 (血管内皮生长因子，vascular endothelial growth factor)真核表达载体，研究VEGF反义RNA表达对人胃癌细胞生物学表型的影响. 方法 用阳性脂质体介导的基因转染技术，将VEGF反义RNA重组体转入人胃癌细胞系，观察转染细胞的细胞周期、增殖能力及致瘤生长情况等生物学表现. 结果 VEGF反义RNA重组体转染胃癌细胞后，斑点杂交、间接免疫荧光等分析显示，反义RNA基因转染技术可使VEGF的表达明显减弱;与未转染的细胞相比，G2 /M期细胞增加(0.39)，而S期细胞减少(0.54);克隆形成率(2.2%)低于未转染细胞(4.0%);肿瘤细胞接种裸鼠后33d时，VEGF反义RNA表达人胃癌细胞所致的移植瘤体积(345±136)mm3 明显小于未转染细胞所致的移植瘤体积(1534±363)mm结论VEGF反义RNA可以通过抑制肿瘤组织血管生成而防治肿瘤的生长，另外VEGF的表达可能与细胞增殖能力有关.

    VEGF165 antisense gene and biological characteristics of human gastric cancer cells

    LIU Du-Hu，ZHANG Xue-Yong，HUANG Yu-Xin，FAN Dai-Ming

    1 Department of Gastroenterology，Tangdu Hospi-tal，Fourth Military Medical University，Xi'an710038，China，

    2 Department of Gastroenterology，Xijing Hospital，Fourth Military Medical Universi-ty，Xi'an710033

    Keywords:stomach neoplasms;vascular endothelial growth factor;antisense nucleotide

    Abstract:AIM To investigate the effect of vascular en-dothelial growth factor(VEGF)antisense gene on the biolog-ical characteristics of human gastric cancer cells by antisense nucleotide technique.METHODS The VEGF165 antisense gene recombinant was introduced into gastric cancer cell line by lipofect transfection technique.Then the cell cycle ......