关键词: 脑损伤;代谢型谷氨酸受体亚型4;病理生理;新武器伤

    中图号:R651.15

    摘 要:目的 研究弥漫性脑损伤(DBI)及其合并二次脑损伤(SBI)后大鼠脑皮层代谢型谷氨酸受体亚型4(mGluR4)的变化及意义. 方法 SD大鼠随机分为正常对照组、假手术组、单纯SBI组、单纯DBI组及合并SBI组.在Marmarou弥漫性脑损伤模型的基础上，制成SBI模型，于伤后1，6，12，24和72h进行HE染色和代谢型谷氨酸受体4亚型(mGluR4)mRNA原位杂交. 结果 与正常对照组相比，假手术组和单纯SBI组阳性神经元数以及信号灰度均无明显改变(P>0.05);与假手术组相比，单纯DBI组和合并SBI组，mGluR4mRNA表达于脑损伤后1h即有明显增加(P<0.01)，单纯DBI组在6h达到高峰，合并SBI组于12h达到高峰，此刻与单纯DBI组相比，合并SBI组亦明显增加(P<0.01). 结论 mGluR4参与了DBI和SBI的病理生理过程，可能为临床救治重型颅脑损伤提供新的理论依据.

    Changes of metabotropic glutamate receptor subtype4mRNA after diffuse brain injuries coupled with secondary brain insults in rats

    BAI Hong-Min，FEI Zhou，ZHANG Xiang，LI Zhi-Gang，LIU En-Yu，LIU Xian-Zhen，LIANG Jing-Wen

    Institute of Neurosurgery of Chinese PLA，Xijing Hospital，Fourth Military Medical University，Xi'an710033，China

    Keywords:brain injuries;metabotropic glutamate receptor subtype4;pathophysiology;new weapon injury

    Abstract:AIM To study the changes and effects of metabotropic glutamate receptor subtype4(mGluR4)after diffuse brain injuries(DBI)coupled with secondary brain in-sults(SBI)in rats.METHODS 105male SD rats were ran-domized into five groups:normal control group ......