关键词: 宁得朗;凋亡;胶质瘤

    中图号:R739.41

    摘 要:目的 探讨宁得朗(NIDRAN)对人脑胶质瘤细胞凋亡的诱导作用. 方法 用含10μmol·L-1 NIDRAN的RPMI1640培养液，作用于SHG44细胞3h，收集细胞用于形态学检测，DNA电泳及流式细胞分析. 结果 ①形态学观察:大部分瘤细胞体积缩小，核固缩，染色质浓缩在核模边缘呈月牙状聚集，有凋亡小体形成.②DNA电泳:在800bp以下可见明显的DNA梯状带.③流式细胞分析:出现典型的细胞凋亡峰，凋亡细胞占细胞总数的32.1%. 结论 NIDRAN可诱导人胶质瘤细胞发生凋亡.

    Study on NIDRAN to induce the apoptosis of human brain glioma cells

    WANG Zhan-Xiang，ZHANG Xiang，ZHANG Yong-Qin，FEI Zhou，FU Luo-An，ZHANG Zhi-Weng，ZHANG Jian-Ning，LIU Wei-Ping

    Institute of Neurosurgery of Chinese PLA，Xijing Hospital，Fourth Military Medical University，Xi'an710033，China

    Keywords:Nimustine hydrochloride;apotosis;glioma

    Abstract:AIM To investigate the effect of Nimustine hy-drochloride(NIDRAN)to induce the apoptosis of human cul-tured glioma cells.METHODS SHG44cells，human glioma cell line，were treated with RPMI1640culture fluid contain-ing10μmol·L-1 NIDRAN for3h.Then these cells were observed morphologically，by DNA electrophoresis and flow cytometry analysis respectively.RESULTS ①The cells be-came smaller，with nucleus of cells shrinking，chromatin aggregating at the margin of nuclear membrane and apoptotic body formed.②DNA ladder under800bp was found by electorphoresis.③The typical apoptotic peak was observed by flow cytomery analysis and apoptotic cells accounted for32.1%.CONCLUSION NIDRAN can induce the apoptosis of human cultured glioma cells. ......