关键词: 角蛋白;角蛋白;逆转录聚后酶链反应;克隆，分子

    中图号:R246.7

    摘 要:目的 克隆并表达人角蛋白K6a cDNA. 方法 从人角质形成细胞中提取RNA，以RT-PCR法扩增人角蛋白K6a(1.7kb)cDNA.测序正确，将该片段克隆入表达载体pRSETB中，用IPTG诱导表达. 结果 获得了角蛋白K6a(1.7kb)cDNA片段，并在大肠杆菌中获得高效表达. 结论 获得了人角蛋白K6a(1.7kb)cDNA片段及其原核表达产物，对研究人角蛋白K6a功能有重要意义.

    Cloning and expression of the cDNA of human ker┐atin6a in E.coli

    ZHANG Hai-Long，WU Cai-Xia，LIU Yu-Feng，GAO Tian-Wen，ZHAO Xiao-Dong

    Center of Dermatology&Venereology of Chinese PLA，Xijing Hospital，Fourth Military Medical U-niversity，Xi'an710033，China

    Keywords:keratinocytes;keratin;reverse transcriptase polymerase chain reaction;cloning，moleculer

    Abstract:AIM To clone and express the1.7kb cDNA fragment of human keratin6a.METHODS The1.7kb cD-NA fragment of human keratin6a was amplified from ker-atinocyte total RNA by RT-PCR，then it was cloned into ex-pression vector pRSETB and induced with IPTG.RESULTS The1.7kb cDNA fragment of human keratin6a was cloned.The sequencing indicated that the sequence was iden-tical to that of reports.Then the1.7kb cDNA was cloned into expression vector pRSETB.The keratin6a fragment was highly expressed in E.coli after induced with IPTG.CONCLUSION The1.7kb cDNA fragment of human ker-atin6a is obtained ......