大鼠延髓和脊髓背角的PKCγ阳性神经元向孤束核投射
蛋白激酶Cγ亚单位,,蛋白激酶Cγ亚单位;延髓背角;脊髓背角;孤束核;伤害性感受;大鼠,1材料和方法,2结果,3讨论,参考文献
关键词: 蛋白激酶Cγ亚单位;延髓背角;脊髓背角;孤束核;伤害性感受;大鼠中图号:R322.81
摘 要:目的 观察延髓和脊髓背角内蛋白激酶Cγ亚单位(PKCγ)阳性神经元向孤束核的投射. 方法 荧光金(FG)逆行追踪与PKCγ的免疫荧光组织化学染色相结合的双标记技术. 结果 PKCγ阳性神经元主要分布于延髓和脊髓背角的Ⅰ,Ⅱ,Ⅲ层及脊髓的外侧脊核;将FG注入孤束核后,在延髓和脊髓背角的Ⅰ,Ⅱ,Ⅲ层及脊髓的外侧脊核内可见FG标记神经元;部分FG标记神经元呈PKCγ阳性,FG/PKCγ双标神经元也主要见于延髓和脊髓背角的Ⅰ,Ⅱ,Ⅲ层及脊髓的外侧脊核. 结论 延髓和脊髓背角的PKCγ阳性神经元可能参与将躯体和内脏伤害性刺激信息向孤束核的传递.
PKCγ┐containing neurons in the medullary and spinal dorsal horns projecting to the rat solitary tract nucleus
WU Li-Ping,LI Hui,LI Yun-Qing,ZHANG Yuan-Qiang
Hui-Bin Wards,Xijing Hospital,Department of Anatomy and K.K.Leung Brain Research Centre,Department of Histology and Embryology,Faculty of Preclinical Medicine,Fourth Military Medical University,Xi'an710033,China
Keywords:PKCγ;medullary dorsal horn;spinal dorsal horn;solitary tract nucleus;nociception;rat
Abstract:AIM To examine the projection of protein kinase Cγisoform(PKCγ)-immunoreactive neurons from the medullary and spinal dorsal horns to the solitary tract nucleus in the rat.METHODS Fluoro-gold(FG)retrograde tracing combined with immunofluorescence histochemical staining for PKCγwas used.RESULTS PKCγ-immunoreactive neurons were observed in laminaeⅠ ......
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