关键词 :肾综合征出血热;汉坦病毒;病毒壳体;表位作图;肽合成;酶联免疫吸附测定

    摘 要:目的 以合成肽为抗原建立新型特异性检测抗汉坦病毒IgG和IgM的ELISA. 方法 用多肽合成仪合成汉坦病毒核衣壳蛋白(aa17-66)50个氨基酸残基的核心序列，以此为抗原，通过实验发现这一序列具有较强的抗原性，首次建立了以合成肽为抗原的新型特异性检测抗汉坦病毒IgG和IgM的ELISA.测定肾综合征出血热(HFRS)患者血清23份，乙型肝炎患者血清9份，正常人血清11份. 结果 乙型肝炎患者血清和正常人血清均为阴性，20份HFRS血清IgG阳性，21份HFRS血清IgM阳性.本方法的相对敏感性为86%和91%，相对特异性为100%，符合率为93%和95%. 结论 本法特异性强，灵敏度高，简便易行，是适于基层医疗单位的诊断方法.

    Preparation of major antigenic epitope of Hantaan virus nucleocapsid protein and its applications

    LI Zheng，BAI Xue-Fan，BIAN Hui-Jie

    1 Department of Chemistry，Shaanxi Normal Univer-sity，710062Xi'an，China，

    2 Department of Infec-tious Diseases，Tangdu Hospital，Fourth Military Medical University，Xi'an710038，China，

    3 Cell Engi-neering Research Centre，Fourth Military Medical University，Xi'an710033，China

    Keywords:hemorrhagic fever with renal syndrome;Hantaan-virus;capsid;epitope mapping;peptide synthe-sis;enzyme-linked immunostorbent assay

    Abstract:AIM To establish a new specific serological diag-nostic method to test HFRS.METHODS Epitope(aa17-66)of nucleocapsid protein was synthesized by solid-phase pep-tide synthesis.Then the synthetic peptide was applied as an antigen to test the specific antibody(IgG ......