Keywords:keratin;psoriasis;peptide library;oligopeptides;bacteriophages;antibodies

    monoclonal CLC number:R758.63

    Abstract:AIM To acquire the mimetic homologous oligopeptides of human epidermal keratins14and17related with psoriasis and evaluate the effect of the oligopeptides on the pathogenesis of psoriasis.METHODS The mAb5G5recognizing the common epitope of human epidermal keratin K14and K17was purified by HiTrap Protein G affinity col-umn，and was biotinylated by the biotin ester.A6-mer phage random peptide library was biopanned for3cycles，then positive clones were identified by ELISA and DNA were extracted for sequencing.RESULTS Amino acid sequence analysis showed that10positive clones selected randomly had the consensus Amino acid sequence(motif)VL(x)AG.The motif VL(x)AG could be detected in the homologous amino acid sequence of keratins14and17，and streptococcal M pro-tein contained the motif too.The phages of positive clones reacted with mAb5G5specifically and prevented the interac-tion between mAb5G5and keratins with dose-dependent ef┐fects.CONCLUSION The motif could mimic the common epitope on human epidermal keratins14 ......