关键词: 反义寡核苷酸;CRKL基因;慢性粒细胞性白血病

    摘 要:目的 通过研究CRKL基因反义寡核苷酸对K562细胞增殖活性的影响，探讨CRKL基因在Ph+ 慢性粒细胞性白血病(CML)发病中的作用. 方法 脂质体为载体，CRKL基因反义寡核苷酸转染K562细胞，通过活细胞记数、H3 -TdR掺入、RT-PCR等方法观察K562细胞的增殖活性及基因表达的变化. 结果 CRKL反义寡核苷酸对K562细胞的增殖有抑制作用. 结论 CRKL基因在Ph+ CML的发病中可能有重要作用，可作为CML治疗的新靶点.

    Effect of CRKL gene expression on cell prolifera┐tion in K562cell line

    WANG Sha，SUN Bing-Zhong，HUANG Gao-Sheng，YU Wen-Qiang，CHAI Yu-Bo

    1 Department of Hematology，Xijing Hospital，2 Department of Pathology，3 Department of Bio-chemistry and Molecular Biology，Faculty of Pre-clinical Medicine，Fourth Military Medical Universi-ty，Xi'an710033，China

    Keywords:antisense-oligonucleutide;CRKL gene;chronic mylocytic leukemia

    Abstract:AIM To investigate the effects of CRKL anti-sense-oligonucleutides(ASODN)on cell growth and prolifer-ation in K562cell line and thus assess CRKL gene in the pathogenesis of Ph+ CML.METHODS K562cells were cul-tivated with CRKL oligos which were mediated by cationin li-posome.The cell viability，proliferation，and gene expres-sion were examined by H 3 -TdR doping and RT-PCR.RE┐SULTS CRKL ASODN could inhibit the viability and prolif-eration of K562cells.CONCLUSION CRKL gene may play a key role in the pathogenisis in Ph+ CML. ......