胃癌 TIMP3 基因启动子甲基化及其蛋白表达的研究
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关志宇, 戴冬秋
胃癌;TIMP3;CpG岛;甲基化关志宇,戴冬秋.胃癌TIMP3基因启动子甲基化及其蛋白表达的研究.世界华人消化杂志2006;14(2)138-143,胃癌TIMP3基因启动子甲基化及其蛋白表达的研究
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关志宇,戴冬秋，中国医科大学附属第一医院肿瘤外科辽宁省沈阳市 110001
关志宇,男,1969-08-20生,辽宁省鞍山市人,2003年中国医科大学肿瘤外科博士生,副主任医师,主要从事胃肠癌转移分子机制与临床应用研究.
国家自然科学基金项目,No. 30271477
教育部留学回国人员科研启动基金项目,No. 2002-247
通讯作者：戴冬秋,110001, 辽宁省沈阳市和平区南京北街155号, 中国医科大学附属第一医院肿瘤外科, 辽宁省胃癌分子病理重点实验室. daidq63@163.com
电话:024-23256666-6227,6228
收稿日期：2005-09-28 接受日期:2005-09-30
Promotermethylation and expression of tissue inhibitor of metalloproteinase 3 genein gastric cancer
Guan Zhi-Yu, Dai Dong-Qiu
Guan Zhi-Yu, Dai Dong-Qiu, Department of Oncology, the First Affiliated Hospital of China Medical University, Shenyang, 110001,Liaoning Province, China
Supported by National Natural Science Foundation of China, No. 30271477, and the Scientific Research Foundation of Education Ministry for the Returned Overseas Chinese Scholars, No.2002-247
Correspondence to:Dr. Dong-Qiu Dai, Department of Oncology, the First Affiliated Hospital of China Medical University, 155 Nanjing Beijie, Heping District, Shenyang 110001, Liaoning Province, China. daidq63@163.com
Received: 2005-09-28 Accepted: 2005-09-30
Abstract
AIM:To analyze the relations among the aberrant methylation of the promoter CpG islands of tissue inhibitor of metalloproteinase 3 (TIMP3) gene, its protein expression, and the clinicopathological features of gastric adenocarcinoma.
METHODS:The methylation status of promoter CpG islands and protein expression of TIMP3 gene in the tumors and the adjacent normal mucosal tissues of 78 patients with gastric adenocarcinoma were detected by methylation-specific PCR (MSP) and immunohistochemistry respectively.
RESULTS:The CpG island methylation of TIMP3 was detected in tumour tissues, cancer-adjacent tissues and lymph node with metastasis. In increasing order of frequency, the hypermethylation rates of these tissues were 35.9% (28 of 78 non-neoplastic tissues), 85% (17 of 20 early-stage cases), 89 ......
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