温热体外对人胃癌细胞株生物学行为的影响
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汤 睿, 朱正纲, 瞿 颖,李建芳, 纪玉宝, 刘
温热;胃癌细胞株汤睿,朱正纲,瞿颖,李建芳,纪玉宝,刘炳亚,燕敏,林言箴.温热体外对人胃癌细胞株生物学行为的影响.世界华人消化杂志2006;14(2)144-151,温热体外对人胃癌细胞株生物学行为的
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汤睿,朱正纲,瞿颖,李建芳,纪玉宝,刘炳亚,燕敏,林言箴, 上海交通大学医学院附属瑞金医院上海消化外科研究所上海市200025
汤睿, 上海交通大学医学院附属第九人民医院普外科上海市200011
汤睿, 1972-08-04生,上海市人, 汉族, 2000年上海第二医科大学硕士毕业, 2003级上海交通大学医学院博士生, 主治医师, 主要从事胃肠道肿瘤综合治疗的临床与基础研究.
上海市科委课题资助项目,No. 044119625
上海市医学重点学科建设课题资助项目,No. 05Ⅲ005
通讯作者:朱正纲,200025, 上海市瑞金二路197号,上海交通大学医学院、上海交通大学医学院附属瑞金医院普外科,上海消化外科研究所.zzg1954@hotmail.com
电话:021-64370045-611003 传真:021-64373909
收稿日期:2005-10-14 接受日期:2005-10-31
Impact of hyperthermia on human gastric cancercell lines in vitro
Rui Tang, Zheng-Gang Zhu, Ying Qu,Jian-Fang Li,Yu-Bao Ji, Bing-Ya Liu, Min Yan, Yan-Zhen Lin
Rui Tang, Zheng-Gang Zhu, Ying Qu, Jian-Fang Li, Yu-Bao Ji, Bing-YaLiu, Min Yan, Yan-Zhen Lin, ShanghaiInstitute of Digestive Surgery, Ruijin Hospital, Medical College ofShanghai Jiaotong University, Shanghai 200025, China
Rui Tang, Departmentof General Surgery, Shanghai Ninth People’s Hospital, Medical College ofShanghai Jiaotong University, Shanghai 200011, China
Supported by the Science and Technology Committee Foundation of Shanghai, No. 044119625; and the Foundation of Key Medical Academic Discipline of Shanghai, No. 05Ⅲ005
Correspondence to:Professor Zheng-Gang Zhu, Department of General Surgery, Ruijin Hospital, Medical College of Shanghai Jiaotong University; Shanghai Institute of Digestive Surgery, 197 Ruijin Er Road, Shanghai 200025, China. zzg1954@hotmail.com
Received: 2005-10-14 Accepted:2005-10-31
Abstract
AIM:To investigate the impact of hyperthermia (HT) on human gastric cell lines in vitro.
METHODS:Six human gastric cancer cell lines (AGS, MKN45, SGC7901, NCI-N87, SNU-1 and SNU-16) were used in this study. HT was achieved by incubating cells at 43 ℃ for 2 h, and cells incubated at 37 ℃ served as normothermia (NT) control. MTT assay was adopted to evaluate cell proliferation and cytotoxicity after HT ......
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