关键词: 活体供者;小肠/移植;白细胞介素8;移植物排斥

    摘 要:目的 观察我国首例活体部分小肠移植受体围手术期趋化因子IL-8的变化及其与移植物缺血再灌注损伤和急性排斥反应的相关性. 方法 双抗夹心ELISA法测定受体术前及术后1～140d血清IL-8动态水平. 结果 受体血清IL-8水平在术后1d显著升高(2.44μg L-1 vs0.26μg L-1 )，且于次日迅速下降(1.70μg L-1 )，术后4d达术前水平.血清IL-8的第2个高峰出现于术后67d发生移植物急性排斥反应时(1.47μg L-1 )，但高度较第1次低，恢复较第1次慢，在术后96d达术前水平. 结论 在人活体部分小肠移植中，血清IL-8水平与移植物缺血再灌注损伤和急性排斥反应密切相关，可作为重要的小肠移植物急性排斥反应的信号之一.

    Serum IL┐8levels correlate with acute rejection in recipient with human living┐related small bowel transplantation

    DONG Guang-Long WANG Wei-Zhong WU Guo-Sheng SONG Wei-Liang JI Gang LI Qi JIN Bo-Quan

    1 Department of Gastrointestinal Surgery，Xijing Hospital，

    2 Department of Immunology，Faculty of Preclinical Medicine，Fourth Military Medical Uni-versity，Xi'an710033，China

    Keywords:living donnors;intestine，small/transplantation;interleukin-8;graft rejection

    Abstract:AIM To investigate the perioperative serum lev-els of IL-8in the first case of living-related small bowel transplantation in China and to study the interrelationship of the changes in serum IL-8level with ischemic and reperfusive injury and with the acute small bowel allograft rejection.METHODS Dynamic serum levels of IL-8of recipient were analyzed by a solid-phase double ligand ELISA method once preoperation and several times during1～140d postopera┐tion.RESULTS Recipient's IL-8serum level increased on1postoperatior day(POD)(2.44μg L -1 vs0.26μg L-1 ) ......