关键词: 木黄酮;垂体腺瘤细胞;体外培养

    摘 要:目的 观察木黄酮(genistein，GST)对培养的人垂体腺瘤细胞增殖的影响. 方法 将GST作用于体外培养的人垂体腺瘤细胞，测定MTT值及3 H-TdR参入量，并用流式细胞仪测定细胞周期. 结果 不同浓度的GST(10-7 ，10-6 和10-5 mol·L-1 )明显抑制垂体腺瘤细胞的增殖，MTT的A490nm 值变化率分别为(91.2±4.9)%，(78.8±5.3)%和(65.8±5.6)%，(P<0.01)，cpm值的变化率分别为(88.6±3.6)%，(75.5±5.7)%和(58.9±4.9)%，(P<0.01)，并存在着剂量效应.10-5 mol·L-1 的GST可使G1期细胞比例从对照组的68.2%上升为91.0%(P<0.01).木黄酮对垂体腺瘤细胞的作用与雌激素受体无关. 结论 GST在体外能明显抑制培养的人垂体腺瘤细胞的增殖.

    Inhibitory role of genistein in proliferation of hu┐man pituitary adenoma cells

    CHI Su-Min ZHOU Shi-Sheng LIU Ya-Li GU Jian-Wen WANG Fu-Zhou

    1 Department of Physiology，Faculty of Preclinical Medicine，

    2 Institute of Neurosurgery of Chinese PLA，Xijing Hospital，Fourth Military Medical U-niversity，Xi'an710033，China

    Keywords:genistein;pituitary adenoma cells;in vitro

    Abstract:AIM To study the influence of genistein on the proliferation，DNA synthesis and cell cycle of cultured human pituitary tumors cells.METHODS MTT method and3 H-TdR incorporation cell cycle analysis were used to examine the changes of proliferation and DNA synthesis of human

    pituitary tumors cells.RESULTS In a dose dependent man-ner ......