关键词: 转化生长因子;肠粘膜/损伤;修复;抗体

    摘 要:目的 动态观察转化生长因子(TGF)在不同肠粘膜病理状态下的表达，探讨TGF在肠粘膜损伤后修复过程中所起的作用. 方法 以巴西日圆线虫(Nippostrongylus bras-iliensis，Nb)感染大鼠建立肠粘膜损伤修复模型，手术取出不同感染阶段大鼠小肠肠段，标本冰冻保存，切片.应用免疫组织化学染色方法检测TGF的表达. 结果 在大鼠肠粘膜感染Nb后发生的损伤至修复过程中，TGFα，TGFβ1的表达发生了明显的规律性的变化，即TGFα先是表达于肠绒毛隐窝区域的上部，接着逐渐向肠绒毛的顶端区域转移;而TGFβ1先是主要表达于肠绒毛的顶端部位及隐窝区域的上部，随之顶端部位的表达逐渐减少，最后主要定位表达于隐窝区域的上部.固有膜内TGFα，TGFβ1也有表达，但粘膜损伤前后及修复过程中，其表达强度及部位无明显变化. 结论 TGFα，TGFβ1在粘膜损伤后可能通过在粘膜上皮细胞表达部位的变化，促进细胞分化、迁移和增殖，与粘膜损伤后修复关系密切.

    Exxpression of transforming growth factorαandβ1in rat small intestine infected with Nippostrongylus brasiliensis

    YAO Wen-Zhu HUJia-Lu WUKai-Chun CUI Da-Xiang

    1 Institute of Gastrointestinology of Chinese PLA，Xijing Hospital，

    2 Institute of Genetic Diagnosis Technology，Fourth Military Medical University，Xi'an710033，China

    Keywords:transforming growth factor;intestinal mucosa/iusures;ristitution;antibody

    Abstract:AIM To investigate the role of TGFαand TGFβduring the all course of the restitution of wounded gastroin-testinal epithelia after injury in vivo.METHODS Rat small intestine was infected with Nippostrongylus brasiliensis(Nb)as the model of epithelia injury-and-restitution.Rat intestinal fragments were obtained surgically and preserved frozen ......