关键词: 成纤维细胞;细胞培养

    摘 要:目的 探讨来自正常皮肤、增生性瘢痕和瘢痕疙瘩组织的成纤维细胞的生物学特性. 方法 对三种组织均采用组织块法进行细胞的原代培养，采用胰蛋白酶消化传代，分别利用绘制细胞生长曲线，3 H-胸腺嘧啶掺入及3 H-脯氨酸掺入等方法观察细胞的增殖，DNA合成代谢及胶原合成等情况，并比较它们之间的差异. 结果 瘢痕疙瘩成纤维细胞在细胞增殖、DNA合成代谢及胶原合成量等方面均明显高于正常皮肤及增生性瘢痕组织来源的成纤维细胞，后两者之间有一定区别但无统计学意义. 结论 不同组织来源的成纤维细胞其生物学行为亦有所区别，因此在进行实验研究时应有一定的针对性.

    Biological characteristics of human skin fibroblasts derived from different tissues

    HAN Jun-Tao，CHEN Bi，TANG Chao-Wu

    Department of Burn Surgery，Xijing Hospital，Fourth Military Medical University，Xi'an710033，China

    Keywords:fibroblasts;cell culture

    Abstract:AIM To investigate the biological characteristics of fibroblasts derived from normal human dermal，hyper-trophic scar and keloid tissues.METHODS Fibroblasts pri-mary culture was carried out by the assay of planting tiny tis-sue masses into culture bottles.The fibroblasts，after being isolated from different tissues，were cultivated and the cell growth curves were drawn.Meanwhile ......