关键词: 神经胶质瘤;杂交;肿瘤相关基因

    摘 要:目的 克隆胶质瘤相关基因. 方法 取胶质瘤患者新鲜肿瘤标本及肿瘤附近正常脑组织提取mRNA，反转录成cDNA.采用基于PCR的消减杂交法获得差异的cDNA，并经多聚酶链反应扩增后克隆入T载体测序.用打点杂交法验证差异克隆. 结果 获得一胶质瘤相关的酸性成纤维生长因子基因同源物. 结论 该酸性成纤维生长因子基因同源物是酸性成纤维生长因子mRNA的不同拼接产物，可能参与胶质瘤的形成.

    Glioma related homolog of acid fibroblast growth factor gene

    DENG Yan-Chun，YAO Li-Bo，WANG Ji-Cun，LIU Xin-Ping，NIE Xiao-Yan，JI Sao-Ping，LI Fu-Yang

    Department of Biochemistry and Molecular Biology，Faculty of Preclinical Medicine，Fourth Military Medical University，Xi'an710033，China

    Keywords:nerve glioma;hybridization;tumor related gene

    Abstract:AIM To search for glioma related genes.METH┐ODS Glioma sample and normal brain tissue of a patient were got under operation.mRNAs were extracted and re-verse transcribed into cDNA.Subtractive hybridization was done to get the glioma-specific cDNA which was amplified by polymerase chain reaction(PCR).The PCR products were cloned into vectors for sequencing.Dot hybridization was used to testify the different clone.RESULTS A homolog of acid fibroblast growth factor(aFGF)mRNA was found in tumor tissue but not in normal brain tissue.CONCLUSION The new gene is the different splicing product of aFGF and might be involved in glioma genesis. ......