关键词: 关节炎，类风湿;滑膜细胞;PCR

    摘 要:目的 筛选类风湿性关节炎(RA)滑膜细胞中的高表达基因，以探讨RA滑膜细胞发生肿瘤样增殖和侵蚀软骨的分子机理. 方法 以骨性关节炎(osteoarthritis，OA)滑膜细胞作对照，采用差示PCR(differentially display PCR)方法，克隆RA滑膜细胞中高表达的cDNAs片段，经反向点杂交排除假阳性克隆后，将阳性克隆进行基因序列分析. 结果 共回收76个差异条带，得到42个有插段的克隆，经反向点杂交后得到22个阳性克隆，对其中19个克隆进行基因序列分析表明，最长片段为501bp长，最短片段为145bp长，大部分在300bp.有13个克隆为已知基因，其中包括组织蛋白酶，其它6个克隆为未知序列，这些未知序列中除一个序列外都为基因的非编码区. 结论 差示PCR方法在筛选差异表达基因方面存在着很大的局限性;组织蛋白酶B可能与RA滑膜细胞的软骨侵蚀性有关.

    Identification of multiple，differentially expressed messenger RNAs in fibroblast┐like synovial cells from patients with rheumatoid arthritis

    WANG Ji-Cun YAO Li-Bo LU Hou-Shan LIU Xin-Ping ZHAO Jin-Rong DENG Yan-Chun SUN Tie-Zheng NIE Xiao-Yan

    1 Department of Biochemistry and Molecular Biolo-gy，Faculty of Preclinical Medicine，Fourth Military Medical University，Xi'an710033，China，

    2 Department of Orthopedics，Renmin Hospital，Bei-jing Medical University，Beijing100044，China

    Keywords:arthritis，rheumatoid;synovial cells;PCR

    Abstract:AIM To simultaneously identify several genes whose expression increase in fibroblast-like synovial cells(FLS)of rheumatoid arthritis(RA)patients and to be relat-ed to the proliferation or invasion of FLS to cartilage of RA patients.METHODS Total RNAs were prepared separately from FLS of RA patients and osteoarthritis(OA ......