关键词: p38分裂原激活蛋白激酶;基因转染;胶质瘤;凋亡;大鼠

    摘 要:目的 研究p38MAPK基因转染大鼠胶质瘤细胞系C6后对其生物学特性的影响. 方法 利用脂质体介导法将p38MAPK基因导入大鼠胶质瘤细胞系C6中，用免疫细胞化学染色检测其在细胞转染前后的表达情况，用HE染色、流式细胞仪等方法研究其对细胞形态、粘着状况和生长周期的影响. 结果 转染pCMV5-p38MAPK质粒组p38MAPK蛋白表达阳性，细胞形态发生变化，贴壁性降低，出现大量凋亡细胞. 结论 转染p38MAPK基因可诱导胶质瘤细胞凋亡.

    p38MAPK induces apoptosis of glioma cell

    ZHANG Bi-Cheng LI Qing GONG Xi-Qi YE Jing CHEN Guang-Sheng WANG Ying-Mei LIN Sheng-Cai

    1 Department of Pathology，Xijing Hospital，Fourth Military Medical University，Xi'an710033，China，

    2 Third Division Headquarters Hospital，Beijing Gar-rison Command，Beijing，

    3 Institute of Molecular and Cell Biology，National University of Singapore，Singapore

    Keywords:p38MAPK;gene transfection;glioma;apoptosis;rats

    Abstract:AIM To study the effect of p38MAPK transfec-tion on the biological characteristics of glioma cell C6.METHODS p38MAPK was transfected into glioma cell C6by lipofectin.Expression of p38MAPK was detected by immunocytochemistry.HE staining and flow cytometry were adopted to measure the cell morphology，adhesion and cell cycle.RESULTS p38MAPK was expressed in transfected glioma cells ......