关键词: 视上核;脑片;全细胞记录;吗啡;突触传递

    摘 要:目的 研究吗啡对大鼠视上核神经元兴奋性突触后电流(excitatory postsynaptic currents，EPSCs)的影响，并对其突触机制进行探讨. 方法 采用脑片膜片钳全细胞记录技术，在电压钳状态下，记录吗啡对大鼠视上核神经元EPSCs和mEPSCs(miniature EPSCs)的影响. 结果 20μmol L-1 的吗啡可分别使大鼠视上核神经元的EPSCs的频率下降65%，幅度减少44%，mEPSCs频率下降45%，幅度减少12%. 结论 吗啡通过突触前机制抑制视上核神经元突触前兴奋性递质的传递.

    Presynaptic inhibitory action of morphine on excita┐tory synaptic transmission in rat SON neurons in brain slices

    WANG Xiao-Bin，HU San-Jue，JU Gong

    Institute of Neuroscience of Chinese PLA，Fourth Military Medical University，Xi'an710033，China

    Keywords:supraoptic nucleus;brain slice;whole-cell recording;synaptil transimission

    Abstract:AIM To observe the effects of morphine on the excitatory postsynaptic currents(EPSCs)and miniature EP-SCs(mEPSCs)in rat supraoptic nucleus(SON)neurons and to determine its synaptic mechanism.METHODS Using whole-cell voltage-clamp recording technique in brain slices，the EPSCs and mEPSCs of rat SON neurons were recorded，respectively.RESULTS 20μmol L-1 morphine decreased the frequency of EPSCs and mEPSCs(EPSCs by65% ......