关键词: 胃肿瘤;癌前状态;基因表达;免疫组织化学

    摘 要:目的 探讨胃癌及其癌前病变中ras，c-erbB-2，p53癌基因蛋白产物的表达分布特点及其在胃癌发生发展中的作用. 方法 应用免疫组化ABC方法，对58例胃癌、31例胃粘膜上皮异型增生和11例正常胃粘膜组织中，鼠抗人mAb ras基因p21蛋白、兔抗人c-erbB-2原癌基因产物多克隆抗体p185，鼠抗人mAb p53蛋白进行了检测. 结果 p21在正常胃粘膜和轻、中、重度异型增生和胃癌中的表达率分别为27.3%，30.0%，46.2%，75.0%和72.4%;其表达率在重度异型增生和胃癌中显著高于正常胃粘膜和轻、中度异型增生(P<0.05);p185在正常胃粘膜和轻、中度异型增生中无表达，在重度异型增生和癌中的阳性表达率分别为25.0%，18.9%，各组间无显著性差异(P>0.05);p53仅在癌中有表达，表达率29.3%，明显高于非癌组织(P<0.05).胃癌中两种以上癌基因蛋白同时表达率明显高于非癌组织(P<0.05). 结论 ras基因可能作用于胃癌发生的早期阶段，c-erbB-2，p53基因可能作用于胃癌发生的较晚期.并认为胃癌发生需要多基因协同作用.

    Expression of ras p21，p53and p185oncoproteins in cancer and precancerous lesions of stomach

    HUI Yan-Ping HUANG Gao-Shen WANG Wen-Liang WANG Ying-Mei ZHU Xiao-Hui MA Fu-Chen

    1 Department of Pathology，Xijing Hospital，

    2 Department of Pathology，Faculty of Preclinical Medicine，Fourth Military Medical University，Xi'an710033，China

    Keywords:stomach neoplasm;procancerous condition;gene expression;immunohistochemistry

    Abstract:AIM To investigate the expression and distribu-tion features of p21，p53and p185oncoproteins in gastric cancer and its precancerous lesions.METHODS Immuno-histochemical method was used to measure tissues of58gas-tric cancer and31gastric mucosa dysplasia.RESULTS Pos-itive rate of p21in mild ......