摘 要:目的 为比较毛圆线虫卵培养和感染性幼虫分离的几种方法。 方法 采用培养中添加锯末，蛭石培养羊粪及分离幼虫用纱布袋浸泡、贝尔曼分虫法、广口罐分离法。 结果 在绵羊粪便毛圆线虫卵的培养中，用锯末作为添加物较蛭石作添加物，幼虫的孵化率提高1.8倍，两者差异显著(P≤0.05);用纱布袋浸泡法进行粪便培养物中感染性幼虫的分离，较贝尔曼分虫法提高3.7倍;较广口罐分离法提高2.6倍。 结论 以上方法分离的感染性幼虫再经蔗糖垫层离心提取后，可得到纯净、高密度的活性虫体。 关键词: 毛圆线虫;虫卵培养;幼虫分离法比较

    A COMPARISON OF EGG CULTIVATION AND INFECTIVE LARVAL ISOLATION OF TRICHOSTRONGYLIDAE FROM SHEEP FAECES

    WANG Zhi-cai，XU Zhi-guang，LI Jian-jun，MALI Ya-mu，WANG Lin

    (1.Institute of Veterinary Medicine，Xinjiang Academy of Animal Science，Urumqi830000，China;2.Animal Medicine college，Xinjiang Agriculture University，Urumqi830052，China)

    Abstract: The larval recovery rate increases1.8times with the additive of sawdust than with vermiculite in cultivation of eggs of Trichostrongylidae from sheep faeces，and the difference is significant(P≤0.05).The isolation rate of infective larvae with the method of gauze bag dip-in is3.7times higher than Baermann method ......