关键词: 超微结构;PC12细胞;凋亡;帕金森病;多巴胺

    摘 要:目的 研究帕金森病(PD)多巴胺能神经元的死亡机制. 方法 在免疫组织化学基础上，采用流式细胞仪、电泳及电镜技术观察不同浓度多巴胺(DA)诱导大鼠嗜铬细胞瘤PC12细胞凋亡的超微结构及生化改变. 结果 适当浓度DA可诱导PC12细胞凋亡.早期表现为线粒体结构及功能的改变并有抗凋亡蛋白bcl-2的表达，后期电镜下可见凋亡特征性核结构改变及典型的DNA“阶梯状”电泳带. 结论 细胞凋亡参与了PD的病变过程，线粒体在早期细胞凋亡中起着重要的调节作用.

    Analysis of immunohistochemistry and ultrastruc┐ture of apoptosis in PC12cell induced by dopamine

    LI Li-Hong，GAO Guo-Dong，WANG Xue-Lian，ZHANG Bao-Guo

    Department of Neurosurgery，Tangdu Hospital，Fourth Military Medical University，Xi'an710038，China

    Keywords: ultrastructure; PC12 cells; apoptosis;Parkinson's disease;dopamine

    Abstract:AIM To study the mechanism of dopaminergic neuronal death of Parkinson's diseases(PD).METHODS Using the flow cytometry，electron microscope，elec-trophoresis and the immunohistochemistry technology，we observed the alteration of ultrastructure and biochemistry of the apoptosis on PC12cells induced by dopamine with differ-ent concentration.RESULTS Moderate concentrations of dopamine(DA)induced apoptosis in PC12cell.The early al-teration was that of structure and function of mitochondrion and the expression of bcl-2anti-apoptosis protein.Then the specific nuclear ultrastructure of apoptosis and the typical DNA“ladder”electrochromatography were found.CON┐CLUSION Apoptosis may be involved in the pathogenesis of PD ......