关键词: 心房颤动;风湿性心脏病;钙;Fluo-3

    摘 要:目的 观察风湿性心脏病(RHD)心房纤颤(AF)患者心房肌细胞内是否存在Ca2+ 超载. 方法 急性分离RHD伴AF和非AF患者的心房肌细胞，用Fluo-3作为钙指示剂，用共聚焦显微镜测定细胞内Ca2+ 浓度. 结果 AF组心房肌细胞内Ca2+ 浓度明显高于非AF组心房肌细胞内Ca2+ 浓度(517±98)nmol L-1 vs(262±65)nmol L-1 ，两组间差异显著(P<0.01). 结论 RHD伴AF患者心房肌细胞内存在Ca2+ 超载.

    Does Ca2+ overload exist in atrial myocytes of rheumatic heart disease patients with atrial fibrilla┐tion?

    ZHOU Geng-Xu LIANG Yan-Chun ZHANG Rong-Qing JIA Guo-Liang CAI Zhen-Jie

    1 Department of Cardiovascular Surgery，Xijing Hos-pital，Fourth Military Medical University，Xi'an710033，China，

    2 Department of Cardiology，Shenyang Command General Hospital，Shenyang110015，

    3 Department of Cardiology，Xijing Hospi-tal，Fourth Military Medical University，Xi'an710033，China

    Keywords:atrial fibrillation;rheumatic heart disease;calci-um;Fluo-3

    Abstract:AIM To observe whether Ca2+ overload exists in atrial myocytes of rheumatic heart disease patients with atrial fibrillation.METHODS Atrial myocytes of rheumatic heart disease patients with and without atrial fibrillation were a-cutely isolated.Intracellular Ca2+ concentration was mea-sured with the fluoresent Ca2+ indicator Fluo-3and laser scanning confocal microscopy.RESULTS Intracellular Ca2+ concentration of atrial fibrillation group was significantly higher than that of non-atrial fibrillation group [(517±98)nmol L-1 vs(262±65)nmol L-1 )].CONCLUSION Ca2+ overload exists in atrial myocytes of rheumatic heart disease with atrial fibrillation patients. ......