关键词: 钙结合蛋白D28k;帕金森病;PCR;原位杂交;小鼠

    摘 要:目的 研究钙结合蛋白D28k(CaBP)在帕金森病(PD)发病机制中的作用. 方法 将1-甲基-4-苯基-1，2，3，6-四氢吡啶(MPTP)按30mg·kg-1 剂量腹腔注射给C57BL小鼠，建立PD鼠模型.用反转录PCR及原位分子杂交方法检测PD鼠和正常鼠脑内CaBP mRNA的表达变化. 结果 与正常鼠对比，CaBP mRNA在PD鼠的海马、纹状体、黑质等脑区的表达水平明显下调. 结论 CaBP可能通过对特定脑区神经元的保护作用来抵御PD的发生.

    Expression of calbindin┐D28k mRNA in related brain regions of Parkinson's disease in mice

    WANG Lian-Gang WU Sheng-Xi WANG Xi-Qing WU Yi-Jun FENG You-Qi

    1 Department of Experimental Surgery，3 Department of Neurology，Xijing Hospital，

    2 Department of Anatomy，Faculty of Preclinical Medicine，Fourth Military Medical University，Xi'an710033，China

    Keywords:Calbindin-D28k;Parkinson's disease;PCR;in situ hybridization;mouse

    Abstract:AIM To study the possible role of Calbindin-D28k(CaBP)in the pathogenesis of Parkinson's disease(PD).METHODS 1-methyl-4-phenyl-1 ......