关键词: 人;受体，白细胞介素1;基因;基因表达;大肠杆菌

    摘 要:目的 获得一株重组人白细胞介素1受体拮抗蛋白的高效表达菌株，为研究各种急慢性炎症疾病的发病机制以及拮抗IL-1的生物学效应奠定基础. 方法 分离人外周血淋巴细胞，提取mRNA，反转录PCR获得人白细胞介素1受体拮抗基因(hIL-1ra)，克隆入大肠杆菌表达载体，从而构建高效表达人IL-1ra的重组菌株. 结果 成功构建了IL-1ra的高效表达菌株(pLDH-hIL1ra)，序列测定与文献报道一致.12%SDS-PAGE分析显示此菌株所表达的目的蛋白产物相对分子质量为23ku，与预期结果相符.光密度扫描重组hIL-1ra蛋白质占细菌总蛋白的30%. 结论 在大肠杆菌中成功地表达了hIL-1ra基因.

    Cloning and high yield expression of human inter┐leukin1receptor antagonist in E.coli

    LU Fan ZHAO Zhong-Liang YIN Ying YANG Hui CHEN Su-Min

    1 Department of Biochemistry and Molecular Biolo-gy，Faculty of Preclinical Medicine，Fourth Military Medical University，Xi'an710033，China，

    2 Central Laboratory，Tangdu Hospital，Fourth Military Medical University

    Keywords:men;receptors，interleukin-1;genes;gene ex-pression;Escherichia coli

    Abstract:AIM To obtain a high expression clone of human interleukin1receptor antagonist(hIL-1ra)，which is likely to be of great importance in the pathogenesis of both acute and chronic inflammatory diseases and a competitive inhibitor of the binding of IL-1to IL-1R.METHODS mRNA was ......