关键词: PCR-TRAP;宫颈肿瘤;端粒，末端转移酶

    摘 要:目的 探讨端粒酶活性在子宫颈癌组织发生发展中的作用及临床应用价值. 方法 采用PCR-TRAP微孔杂交法检测45例子宫颈癌组织标本和8例正常的子宫颈组织的端粒酶活性. 结果 45例宫颈癌组织中端粒酶阳性表达率为86.6%，其中宫颈癌Ⅰ期27例，阳性率77.7%，宫颈癌Ⅱ期12例，阳性率100%，宫颈癌Ⅲ期6例，阳性率100%.8例正常宫颈组织中未检测出端粒酶阳性者. 结论 端粒酶活性表达在子宫颈癌的发生发展中起重要的作用，端粒酶活性检测对指导宫颈癌的诊断和治疗具有重要意义.

    Detection of activity of telomerase in cervical carci┐noma tissues

    SHI Mei SU Ming-Quan YANG Xiu-Zhi MA Hai-Xin ZHANG Ge-Meng

    1 Department of Radiotherapy，

    2 Department of Lab-oratory Examination，

    3 Department of Obstetrics and Gynecology，Xijing Hospital，Fourth Military Med-ical University，Xi'an710033，China

    Keywords:PCR-TRAP;cervix neoplasms;telomerase

    Abstract:AIM To investigate the role of telomerase activity in the occurrence and development of cervical carcinoma and its clinical value.METHODS PCR-TRAP was employed to detect the activity of telomerase in45cervical carcinoma spec-imens and8normal cervical neck tissues.RESULTS Rate of telomerase positive expression in45cases of cervical carcino-ma was86.6%.Nine cases fell into phaseⅠof cervical car-cinoma ......