关键词: 促性腺素释放激素(GnRH);受体mRNA;原位杂交;胰脏;大鼠

    摘 要:目的 研究大鼠胰腺促性腺激素释放激素(GnRH)受体mRNA的存在及细胞定位. 方法 采用原位杂交组织化学法. 结果 胰脏的外分泌部腺上皮细胞、胰导管上皮细胞及内分泌部胰岛细胞内均可检测到较强的GnRH受体mRNA杂交信号.杂交阳性信号物质均分布在胞质内，胞核呈阴性. 结论 大鼠胰脏内、外分泌部均能合成GnRH受体.GnRH也是一种消化激素，它由胰脏自身合成，又作用于胰脏，可能参与胰脏内、外分泌功能的调节.

    In situ hybridization of gonadotropin releasing hor┐mone receptor mRNA in rat pancreas

    PU Ruo-Lei1 ，HUANG Wei-Quan1 ，JI Qiu-He1 ，YAO Bing1 ，SUN Lan1 ，ZHANG Rong-Qin2 ，WANG Lei2

    1 Department of Histology and Embryology，Faculty of Preclinical Medicine，Fourth Military Medical U-niversity，Xi'an710033，China，

    2 Department of Bi-ology，Qinghua University，Beijing100084，China Keywords:GnRH;receptor mRNA;in situ hybridization;pancreas;rats

    Abstract:AIM To study whether gonadotropin releasing hormone receptor(GnRHR)mRNA exists in rat pancreas and its cellular localization.METHODS In situ hybridiza-tion with digoxigenin-labeled oligonucleotide probes was adopted.RESULTS Pancreatic endocrine islet cells，ex-ocrine glandular cells and exocrine duct epithelial cells were all found to have GnRHR mRNA hybridization signals.The signals distributed in cytoplasm of all positive cells with nega-tive nucli.CONCLUSION Both of the exocrine and en-docrine cells of the pancrea can produce GnRH recepor.GnRH is also a kind of digestive hormone produced by pan-crea and is responsible for modulating the exocrine and en-docrine function. ......