关键词: 葛根素;脊髓;缺血再灌注损伤;兔

    摘 要:目的 研究葛根素对兔脊髓缺血再灌注损伤的作用. 方法 20只雄性新西兰大白兔随机分成缺血组(A组，n=10)及葛根素组(B组，n=10)，夹闭腹主动脉肾下段20min，建立兔脊髓腰尾段缺血模型.B组于夹闭前10min静脉注射葛根素30mg kg-1 ，A组则静脉注射等容量生理盐水.测定夹闭前、后及再灌注后血浆中丙二醛(MDA)浓度及超氧化物歧化酶(SOD)活性;再灌注4，8，12，24及48h分别对动物后肢运动功能评分;再灌注48h后，处死动物，制作切片，观察其组织病理学变化. 结果 缺血及再灌注后B组MDA值明显低于A组(P<0.05)，而SOD活性明显高于A组(P<0.05);再灌注后4，8，12，24及48h时的神经功能评分B组明显高于A组(P<0.05);光镜下，与A组相比，B组脊髓组织损伤显著减轻，形态基本正常的前角细胞较多. 结论 葛根素对兔脊髓缺血再灌注损伤有显著的保护作用.

    Protective effect of puerarin on spinal cord injury resulting from ischemia and reperfusion in rabbits

    SANG Han-Fei ZHANG Ying-Min XU Li-Xi-an WANG Qiang JI Gen-Lin WU Ming-Chun

    1 Department of Anesthesiology，Xijing Hospital，

    2 Department of Anesthesiology，Stomatological Col-lege，Fourth Military Medical University，Xi'an710033，China

    Keywords:puerarin;spinal cord;ischemic and reperfusion injury;rabbit

    Abstract:AIM To study the effects of puerarin on spinalcord injury resulting from ischemia and reperfusion.METH┐ODS Twenty male New Zealands white rabbits were ran-domly divided into two groups:ischemia group(Group A ......