关键词: 当归多糖;多糖硫酸酯;脾细胞增殖反应

    摘 要:目的 合成不同取代度的当归多糖硫酸酯;探讨氯磺酸对当归多糖硫酸酯化过程的影响和当归多糖及其硫酸酯对小鼠脾细胞增殖反应的作用. 方法 采用氯磺酸-吡啶法进行硫酸化修饰;BaSO4 比浊法测定修饰物的硫含量;MTT比色法研究当归多糖及其硫酸酯对小鼠脾细胞增殖反应的影响. 结果 得到8种不同取代度的当归多糖硫酸酯，硫含量为93.7～171.4mg g-1 ，产率为0.737～0.884g g-1 .当归多糖及其硫酸酯在剂量为500mg L-1 和1500mg L-1 时，给药组光密度明显高于对照组(P<0.01)，作用与剂量有良好的正相关性. 结论 提高氯磺酸的比例，可以提高产物的硫含量，但收率降低.当归多糖及其硫酸酯对活化的T细胞增殖有直接促进作用.

    Synthesis of Angelica Sinensis polysaccharide sulfate and their effects on splenocyte proliferation in vitro

    YANG Tie-Hong，JIA Min，SHANG Peng，MEI Qi-Bing，ZHAO De-Hua

    Department of Pharmacology，Faculty of Preclinical Medicine，Fourth Military Medical University，Xi'an710033，China

    Keywords:Angelica Sinensis polysaccharide;polysaccharide sulfate;splenocyte proliferation

    Abstract:AIM To synthesize Angelica Sinensis polysaccha-ride sulfates with different sulfur content;to explore the ef-fect of chlorosulfonic acid on sulfation in an excess of pyridine and the action of Angelica Sinensis polysaccharide sulfates(APSs)on mouse splenocyte proliferation.METHODS An-gelica Sinensis polysaccharide(AP-Ⅰ)was sulfated by chlorosulfonic acid-pyridine method;the ester sulfate was estimated turbidimetrically as barium sulfate;the effects of APSs on mouse splenocyte proliferation were studied by MTT assays in vitro.RESULTS Eight products with differ-ent sulfur content were obtained.The yield was0.737～0.884g g-1 ......