关键词: 保存，生物学;抗原性;移植，同种;主动脉;主要组织相容性抗原Ⅰ类

    摘 要:目的 探讨液氮低温保存对同种异体主动脉移植物抗原性的影响. 方法 采用细胞培养技术和流式细胞仪技术研究液氮低温保存对大鼠主动脉平滑肌细胞和成纤维细胞主要组织相容性抗原Ⅰ类(MHCⅠ类抗原)表达的影响.建立大鼠同种异体主动脉移植模型，采用免疫组织化学实验研究新鲜主动脉和低温保存的主动脉同种异体移植后MHCⅠ类抗原和MHCⅡ类抗原表达的区别. 结果 正常培养的大鼠主动脉平滑肌细和成纤维细胞MHCⅠ类抗原表达的阳性率分别为(88.7±4.3)%和(84.8±2.0)%;液氮低温保存的大鼠主动脉平滑肌和成纤维细胞在复苏后MHCⅠ类抗原表达的阳性率分别为(89.4±3.5)%和(81.9±2.7)%.统计学分析表明大鼠主动脉平滑肌细胞和成纤维细胞的MHC I类抗原表达在液氮低温保存前后无显著性差别.免疫组织化学实验研究表明新鲜主动脉和低温保存的主动脉同种异体移植后MHCⅠ类抗原和MHCⅡ类抗原表达无显著的差异. 结论 液氮低温保存对大鼠主动脉平滑肌细胞和成纤维细胞MHC类的抗原性没有明显的影响.

    Does fluid nitrogen cryopreservation act on MHC antigenicity of aorta of rats?

    YANG Guang CAI Zhen-Jie ZHANG Tie-Zhu WANG Zeng-Wei

    1 Department of Cardiovascular Surgery，Xijing Hos-pital，Fourth Military Medical University，Xi'an710033，China，

    2 Department of Cardiovascular Surgery，General Hospital of Shenyang Command Area of Chinese PLA

    Keywords:preserration，biological;antigenicity;transplan-tation，homologous;aortic;histocompatibility antiqens clussⅠ

    Abstract:AIM To investigate the effect of fluid nitrogen cryopreservation on the MHC antigenicity of aortic homo┐grafts.METHODS By means of cell culture and flow cy-tometry we studied the effect of fluid nitrogen cryopreser-vation on the expression of MHCⅠantigen of smooth mus-cle cells and fibroblasts of aortic homografts of rats.With the establishment of animal models of aortic homograft trans-plantation ......