[摘 要] 目的 探讨增加自体脾组织移植量的方法，提高自体移植脾组织的功能。 方法 贵州小型香猪3头，经肌注麻醉后剖腹切除脾脏，取脾脏的1.2约150g，切成1mm×1mm×1mm小块备用。在距Treitz韧带50cm处，切取保留肠系膜血管的空肠30cm旷置，再吻合肠管恢复肠腔通道，缝闭肠系膜裂口。旷置空肠等分2段，去除肠粘膜，缝闭一端，将约50g脾组织块分别植于2段肠浆肌膜腔内，缝闭另一端口，妥善固定于肠系膜上。大网膜内移植30g脾组织作为对照观察。 结果 4个月饲养期间内无肠梗阻发。3段肠浆肌膜腔内脾组织再生良好，组织结构与大网膜内移植脾组织无明显差别，2段再生较差，1段形成脓肿。 结论 肠浆肌膜腔内移植脾组织的量较大，可作为自体脾组织的移植术式。

    [关键词] 小肠;脾组织移植;猪

    Splenic autotransplantation to small intestine without mucous membrane in swine

    JIANG Deng-jin，GUO Guang-jin，ZHANG Tian-fei，LEI Yan，ZUO Yan-fang

    Department of Surgical Applied Anatomy and Opera-tive Surgery，College of Medicine，Third Military Medical University，Chongqing400038，China

    Abstract:Objective To explore more effective measure for splenic autotransplantation to enhance the function of splenic autotransplanted tis-sue.Methods Splenectomy on3Guizhou swines was first performed in sterile conditions under general anesthesia by submuscular injection of3%pentobarbital.A half of the spleen about150g from each animal was cut into pieces(1mm1mm1mm)for transplantation.Two15-cm long seg-ments of proximal jejunum at the distance of50cm from Treitz ligment were isolated on their mesentery with intact circulation.Bowel continuity was re-established by end-to-end anastomosis and the mesentery was sutured.The mucosal layer was removed from the isolated segments of small intestine and splenic pieces were implanted into the prepared isolated seromuscular loops of small intestine.The ends of the isolated bowel segments were closed.The bowel segments were fixed to the nearby mesentery to prevent torsion of their vascular pedicles.The splenic pieces about30g were im-planted in greater omentum as control.Results No intestinal obstruction was observed in4months after transplantation.Implanted pieces of splenic tissue regenerated well in3bowel segments ......