关键词: 心肌梗塞;心室肌;钙;β-阻滞剂;比索洛尔

    摘 要:目的 观察心肌梗死(MI)后应用比索洛尔的保护作用与心室肌细胞内钙离子([Ca2+ ]i )的关系. 方法 建立兔MI模型，采用钙敏感性荧光探针Fluo-3为[Ca 2+ ]i 指示剂，应用激光扫描共聚焦技术测定MI后急性分离的非梗死区心室肌[Ca2+ ]i 及比索洛尔对其影响. 结果 对细胞负载Fluo-3/AM后的细胞内游离Ca2+ 图像观察发现，MI心室肌细胞荧光强度、密度明显强于Sham组及β-B组(P<0.001及P<0.05).Sham组、MI组及β-B组心室肌细胞的平均荧光强度分别为293±72，670±131，402±92. 结论 MI后非梗死心室肌细胞内Ca2+ 超载，比索洛尔治疗3wk可减轻心室肌细胞内Ca2+ 超载.

    Changes of intracellular calcium in ventricular my┐ocytes after myocardial infarction in rabbit and the effect of bisoprolol

    LIU Bing，JIA Guo-Liang，ZHANG Rong-Qing，ZHANG Jin-Xia，XU Kai，CHENG He-Xiang，LI Fei

    Department of Cardiovasology，Xijing Hospital，Fourth Military Medical University，Xi'an710033，China

    Keywords:myocardial infarction;ventricular myocyte;calci-um;βblocker;bisoprolol

    Abstract:AIM We investigated changes in intracellular cal-cium([Ca 2+ ]i )of ventricular myocytes after myocardial in-farction and effect of bisoprolol.METHODS Myocardial in-farction model was made in rabbit.[Ca 2+ ]i of isolated rabbit ventricular myocytes was measured with fluorescent indicator Fluo-3and laser scanning confocal microscopy.RESULTS Fluorescence intensity ......