关键词: 受体，LHRH;甲状腺肿瘤;免疫组织化学

    摘 要:目的 探讨人甲状腺肿瘤是否存在促性腺激素释放激素受体(GnRH-R)及细胞定位. 方法 从我院普通外科收集病例标本，分为甲状腺腺瘤组和甲状腺癌组，以瘤旁甲状腺及正常甲状腺为对照，采用兔抗GnRH抗独特型多克隆抗体的免疫组织化学ABC法，确定GnRH-R在甲状腺肿瘤中的定位，并结合图像分析检测GnRH-R在两种肿瘤中相对含量的变化. 结果 甲状腺腺瘤及甲状腺癌细胞均呈较强的GnRH-R免疫反应阳性，阳性物质分布在胞质，胞核呈阴性.甲状腺瘤的阳性信号强于甲状腺癌.瘤旁甲状腺及正常甲状腺滤泡上皮细胞免疫反应阳性较弱，胞质深染，呈棕褐色，胞核淡染，未见有阳性物质分布.图像分析结果，甲状腺瘤组单位面积免疫反应阳性物质的相对含量为0.074±0.013，甲状腺癌组为0.033±0.011，甲状腺瘤组相对含量显著高于甲状腺癌组(P<0.05).瘤旁及正常甲状腺组为0.018±0.007，其相对含量较肿瘤组低(P<0.05). 结论 人甲状腺肿瘤中有GnRH-R存在.GnRH-R含量的多少可能与甲状腺肿瘤的分化程度相关.我们推测GnRH可能参与甲状腺肿瘤发生的调节.

    Immunohistochemical study of gonadotropin┐releas┐ing hormone receptor in human thyroid tumor

    ZHOU Jie，JI Qiu-He，HUANG Wei-Quan，ZHANG Nan-Yan

    1 Department of Endocrinology，Xijing Hospital，2 Department of Histology&Embryology，Fourth Military Medical University，Xi'an710033，China

    Keywords:Receptors，LHRH;thyroid neoplasms;immuno-histochemistry

    Abstract:AIM To study presence of gonadotropin-releasing hormone receptor(GnRH-R)in human thyroid tumor and its cellular location.METHODS Immunohistochemical ABC method and image analysis were used to study the expression and location of Gn RH-R in human thyroid adenoma and car┐cinoma.RESULTS Partial cells of thyroid adenoma and car-cinoma showed immunoreactivity.The reactive substances were distributed in cytoplasm of all positive cells with im-muno-negative nuclei.The staining intensity in thyroid ade-noma was stronger than that in thyroid carcinoma.And nor-mal thyroid follicular epithelia cells adjacent to tumor and normal thyroid follicular epithelia cells showed GnRH-R im-munoreactivity too.The cytoplasm was dark brown while the nuclei showed no staining.Image analysis suggested that rel-ative quantification of GnRH-R was0.074±0.013 ......