葛根素对肾缺血/再灌注时肾小管上皮细胞cFos蛋白表达的影响
葛根素,,葛根素；缺血,再灌注；肾；cFos蛋白,1材料与方法,2结果,3讨论,参考文献
摘要：目的 从细胞凋亡角度研究葛根素治疗肾缺血/再灌注损伤的机制。方法 用肾动脉钳夹法建立大鼠肾缺血再灌注损伤模型，治疗组于肾缺血/再灌注前应用葛根素30mg/kg静脉注射；采用原位末端标记法、免疫组织化学法检测缺血再灌注不同时间点肾小管上皮细胞凋亡及cFos蛋白表达的变化。结果 葛根素能减少细胞凋亡，下调cFos蛋白的表达。结论 葛根素对急性肾缺血/再灌注损伤具有保护作用，其机制与下调凋亡相关基因cFos的表达有关。关键词：葛根素；缺血/再灌注；肾；cFos蛋白
Effects of puerarin injection on cFos protein of renal tubule cells
during ischemicreperfusion in kidneyFu Rongguo, Wang Zhongdong, Zhou Lin, Zhang Hongli,Ma Liqun, Zhang Xingying, Ma Xiaoqin
(1. Department of Nephrology, Second Hospital of Xian jiaotong University, Xian 710004; 2. ICU of Kunlun Factory Hospital, Xian 710004, China)
ABSTRACT: Objective To investigate the role of puerarin on acute renal ischemiareperfusion injury and its mechanism. Methods Acute renal ischemiareperfusion iniury rat model was established by placing the atraumatic vascular clamp in renal pedical for 30 minutes. Rats in puerarin group were given puerarin injection 30mg/kg before ischemia. Immunohistochemical methods were used to detect the changes of expression of cFos proteins; pathomorphological changes of renal ischemiareperfusion injury were alse observed, and the occurrence of apoptosis was detected by TUNEL method. Results The pathological changes mainly occurred in the tubular epithelial cells of rat kidneys.The apoptosis index increased in the kidneys of ischemiareperfusion group and puerarin group,and peaked at 48 h of reperfusion(P<0.01). cFos protein mainly expressed in renal distal convoluted tubules, but the expression of cFos in puerarin group was still less than that in the ischemiareperfusion group. In control group, apoptosis of renal tubule cells and cFos protein expression was little. Conclusion Chinese herb puerarin can protect kidney against ischemia after acute renal ischemiareperfusion injury, which may be associated with decreased cFos protein expression in rats. ......
您现在查看是摘要页，全文长 8825 字符。