关键词: 缩胆素;Oddi括约肌;钙动员;细胞培养

    摘 要:目的 探讨CCK引起兔Oddi括约肌细胞收缩的钙动员机制. 方法 取新西兰兔Oddi肌组织，进行原代细胞培养.将培养的4-7代细胞行Fluo-3/AM负载，用激光共聚焦显微镜测量不同拮抗剂对CCK诱导的细胞内Ca2+ 增加的影响. 结果 不加拮抗剂10-7 mol·L-1 CCK使细胞内Ca2+ 增加(130±43)%，用EGTA+维拉帕米阻断细胞外钙离子内流时，CCK诱导细胞Ca2+ 增加(104±23)%，而用肝素时细胞内Ca2+ 增加值为(23±19)%，加入普鲁卡因后细胞内Ca2+ 增加(85±37)%.同其他组对比，肝素明显抑制了CCK诱导的Ca2+ 升高(P<0.01). 结论 CCK通过动员细胞内IP3 敏感钙库的钙离子引起Oddi细胞Ca2+ 升高，该途径可以被肝素所抑制.

    Pathway of cholecystokinin inducing Ca2+ mobiliza┐tion in rabbit Oddi's sphincter muscle cells

    WANG Xin-Jiang，WEI Jing-Guo，WANG Chun-Mei，WANG Yao-Cheng，HUANG Hai-Dong，WU Qiu-Zhen，MA Fu-Cheng

    1 Department of Radiology，Tangdu Hospital，Xi'an710038，China，2 Department of Electron Micro-scope，Faculty of Preclinical Medicine，3 Department of Radiology，4 Department of Pathology，Xijing Hospital，Fourth Military Medical University，Xi'an710033，China

    Keywords:cholecystokinin;sphincter of Oddi;calcium mobi-lization;cell culture

    Abstract:AIM To demonstrate the mechanism of the calci-um mobilization pathway in rabbit Okki's sphincter muscle cells induced by cholecystokinin(CCK).METHODS Rabbit Oddi's sphincter muscle cells was cultured and loaded with Ca2+ indicator fluo-3/AM ......