关键词: 先天性心脏病;心脏组织;人细小病毒B19;风疹病毒;基因

    摘 要:目的 探讨HPVB19和RV等病原感染与先心病的发病关系. 方法 采用PCR，RT-PCR及ISH等分子生物技术，对79例先心病和46例非先心病的先心病心肌组织中HSVB19，RV，TOX等病原基因进行检测及B19病毒在心肌细胞内定位研究. 结果 ①在先心病心肌组织中B19，TOX，RV，HSV病原基因的检测阳性率分别为18%(12/66)，13%(10/79)，23%(7/30)和4%(3/79)，前三者与对照组间比较有显著性差异(P<0.05)，而HSV在两组间无统计学意义(P>0.05)，提示B19，TOX，RV为先心病发病的重要危险因子.②对活检心肌组检测上述病原基因的阳性病例，同时作血清中B19，TOX，RV的特异性IgM检测均阴性.③通过原位杂交显示B19病毒DNA主要分布在心肌细胞核内，光学显微镜下心肌细胞无炎性浸润. 结论 先心病心肌组织中可检测到HPVB19，TOX，RV基因，并有较高的阳性率，提示它们可能是先心病发病的重要感染病原.

    Detection of HPVB19and RV gene in cardiac tissue from children with congenital heart disease

    ZHANG Guo-Cheng，WANG Xiao-Ming，XU Dong-Liang，SUN Xing，Li Biao

    Department of Pediatrics，Xijing Hospital，Fourth Military Medical University，Xi'an710033，China

    Keywords:congenital heart disease;cardiac tissue;human parvovirus B19;Rubella virus;gene

    Abstract:AIM To explore relationship of HPVB19，Rubel-la virus(RV)and other pathogeny infection with CHD.METHODS HPVB19，toxoplasma(TOX)，RV，Herpes simplex virus(HSV)and other pathogenic gene were detect-ed by PCR，RT-PCR and B19viral location was studied in cardiac tissues by ISH in79cases of CHD and46cases with-out CHD of children.RESULTS ①The positive rate of B19 ......