关键词: 恒磁场;人;血管平滑肌细胞;增殖

    摘 要:目的 探讨恒磁场对离体人脐动脉平滑肌细胞(VSMC)增殖的影响. 方法 将培养至3～10代的人VSMC，分别置于磁感应强度为10，50Gs的恒磁场中培养48h，用MTT法和

    3 H-TdR掺入法检测实验组和对照组VSMC的增殖数量，流式细胞仪分析细胞周期. 结果 在恒磁场的作用下，实验组人脐动脉VSMC的增殖数量在10Gs(0.565±0.096和349±82)和50Gs(0.642±0.092和387±91)显著低于对照组(0.716±0.048和427±86)(P<0.05)，而且阻止平滑肌细胞由静止期(G0 /G1 期)进入DNA合成期(S期)和有丝分裂期(G2 /M期). 结论 平均磁感应强度为10，50Gs的恒磁场抑制人脐动脉VSMC增殖，磁疗对PTCA术后冠脉再狭窄可能具有防治作用.

    Inhibiting effects of constant magnetic fields on the proliferation of human umbilical arterial vascular smooth muscle cells

    FENG Xu-Yang，JIA Guo-Liang，ZHANG Rong-Qing，LI Yan，ZHAO Xin-Guo，CAO Fen，CHENG He-Xiang

    Department of Cardiovasology，Xijing Hospital，Fourth Military Medical University，Xi'an710033，China

    Keywords:constant magnetic field;human;vascular smooth muscle cells;proliferation

    Abstract:AIM To explore the effects of constant magnetic fields(CMF)on the proliferation of human umbilical arterial vascular smooth muscle cells(VSMC).METHODS The VSMC of human umbilical artery which were cultured for3～10posterities were put into the CMFs with10，50Gs for48h.The amount of VSMC proliferation was determined by MTT and ......