【摘要】 目的 探讨水蛭对热刺激后动脉管壁增厚的干预效果。方法 使用局部热刺激兔耳，温度为(43±0.5)℃，每次3h，每天1次,共做3次。模型组分别于造模后1周、2周、4周时取材；水蛭组实验前3天及实验后一直用水蛭灌胃，0.3g/(kg·d)，分别于造模后1周、2周及4周时取材。采用光学显微镜及图像分析仪进行形态学观察。结果 模型组热刺激后动脉中膜成分增殖并突入血管腔内，造成管腔变狭。 造模处平滑肌细胞层数及中膜厚度增加。水蛭组与模型组相比，增殖程度减小,各项观测指标均有不同程度的变化，但统计学分析差异无显著性。结论 0.3g/(kg·d)剂量的水蛭对热刺激后血管壁增厚的干预效果不理想。

    【关键词】 动脉；狭窄；水蛭；形态学

    The intervene effect of Hirudo of the thicken of the vascular wall after heat stimulation

    QU Bo，ZHAO Ai-ming，CHEN Bei-yang，et al.

    The Research Room of the Histoloembryology,Hunan TCM College,Changsha 410007,China

    【Abstract】 Objective This study was designed to build a new experimental model of proliferation of arterial smooth muscle cells(SMC).Methods The central artery of the rabbit ear was heated by(43±0.5)℃ for 3 hours a day，and for three days.We examined the morphological changes of the arterials by light microscopy and digital planimetry，3 days，1 week，2 weeks，and 4 weeks after experiment. Hirudo powder was administered 3 days before heating，and rabbits were examined 4 weeks after experiment.Results As the increasing of the time ......