关键词: 甘草甜素;甘草次酸;色谱法，高压液相;血浆

    摘 要:目的 建立一个同时测定甘草甜素、甘草次酸血浆浓度变化监测的方法，进一步用于“脾主药动学”假说的验证. 方法 采用内标定量的反相高效液相色谱法，以甲醇-水-醋酸(88∶11∶1)为流动相，ODS-3柱(150mm×4.6mm，5μm)为固定相，在紫外250nm检测. 结果 GL，GA在此线性范围内，线性关系良好、精密度较高. 结论 本方法具有简便、灵敏、快速而准确的特点，适用于注射GL制剂或口服中药复方前后GL和GA的体内、体外的药代动力学研究，为进一步进行中药复方的药物监测奠定了基础.

    Simultaneous measurement of glycyrrhizin and gly┐cyrrhetinic acid in rat serum by RP┐HPLC method

    WANG Li-Li ，REN Ping ，HUANG Xi ，ZHANG Li ，HUANG Jing-Qun ，CHEN Jian-Zong ，CHEN Xiao-Li

    1 Laboratory of Clinical Pharmacology of Chinese Materiae Medica，2 Department of Geriatrics，4 Department of Pharmacy，5 Department of Tradi-tional Chinese Medicine，Xijing Hospital，Fourth Military Medical University，Xi'an710033，China， 3 252Hospital of Chinese PLA

    Keywords:glycyrrhizin;glycyrrhetinic acid;chromatogra-phy，high pressure liquid;plasma

    Abstract:AIM Establishment of method for measuring plasma concentration of the glycyrrhizin and glycyrrhetinic acid simultaneously by RP-HPLC，in order to testify the hy-pothesis of pharmacokinetics by traditional Chinese medicine.METHODS RP-HPLC with internal labeling was employed.Methanol-H2 O-acetic acid(88∶11∶1)was used as mobile phase and ODS-3(150mm×4.6mm ......