【摘要】 目的 探索研究水溶性维生素(维生素B1、维生素B6、维生素B12和维生素C)对体外小鼠淋巴细胞活化的作用。方法 采用改良的MTT法，来检测体外小鼠淋巴细胞活性。结果 VitB1 100μl(含0.005μg)、VitB6(12.5μl含0.000625μg)对体外小鼠淋巴细胞活性是明显的，VitC 25μl(含0.01μg)作用也是明显的。VitB12则随浓度增高作用稍有增强，但差异无显著性。结论 结果显示VitB1、VitB6和VitC对小鼠淋巴细胞活性有增强作用。

    【关键词】 水溶性维生素；MTT法；小鼠淋巴细胞

    To use of water solable vitamins for studing the activation of in vitro mice lymphocytes

    SUN Ming-jie, GAO Yan, WANG Ke, et al.

    Department of Immunology,Capital University of Medical Sciences, Beijing 100069，China

    【Abstract】 Objective We proposed to study the effecte of water solule Vitamins(Vitamin B1、Vitamin6、Vitamin12 and Vitamin C)in vitro mice lymphocytes.Methods We took the modified method of MTT to explose the activation or in vitro mice lymphocytes.Results VitB1 100μl(contain 0.005μg)、VitB6(12.5μl contain 0.000625μg)、stimualated the effect of in vitro mice lymphocytes which was obvious and VitaminC(25μl contain 0.01μg)) was manifested too.Conclusion Water solule Vitamins (VitB1、VitB6、VitB12 and VitC)were able to stimulate the ativation of in vitro mice lymphocytes under cerftain concentration. ......