藏药独一味两种方法提取组分对小鼠镇痛作用的影响
閸忔娊鏁拠宥忕窗独一味,,独一味;提取组分;镇痛,0引言,1材料和方法,2结果,3讨论,【参考文献】
Analgesic effects of different extraction components from Lamiophlomis rotata (Benth.) kudo, a medicinal plant in XiZang (Tibet) in mice
LIN TianMu, GU Yi, FANG KunQuan, ZHANG SanQi, JIANG YongPei
Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xian 710033, China
【Abstract】 AIM: To explore an experimental method for the study of effective components in traditional Chinese medicine by chemical separation technology integrated with pharmacological experimentation. METHODS: Lamiophlomis rotata, a medicinal plant in Tibet, was chosen in our study and different compounds were separated by two methods: extraction with solvent and gradient elution with macroporous adsorptive resin. Effective components of analgesic were screened by acetic acid writhing and hot plate test in mice. RESULTS: Samples separated by the two methods had obvious analgesic action on writhing reaction by acetic acid in mice and could prolong the latencies of paw licking to gradient elution with macroporous adsorptive resin. Nbutanol extract, petroleum ether extract and water extract were the main effective components of analgesic in extraction with solvent. 300 mL·L-1 ethanol, 900 mL·L-1 ethanol elution parts and precipitate were the effective components of gradient elution with macroporous adsorptive resin. CONCLUSION: Experimentation on effective components of traditional Chinese medicine has been conducted and effective analgesic components are found in Lamiophlomis rotata (Benth.) kudo. ......
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