Effects of Qidantongmai tablets on the equilibrium between NO and ET, 6ª²ketoª²PGF1¦Áand TXB2 in plasma of dogs with myocardial ischemia reperfusion injury

    MA Aiª²Ling,WANG Zongª²Ren, LI Junª²Chang, HANG Li, ZHENG Jin, WANG Wen, LI Jingª²Hua

    Department of Traditional Chinese Medicine, Xijing Hospital, Fourth Military Medical University, Xi¬ðan 710033, China

    ¡¾Abstract¡¿ AIM: To investigate the effects of Qidantongmai tablet (QDTMT) on the equilibrium between ET and NO and the equilibrium between TXB2 and 6ª²ketoª²PGF1¦Áin the canine model of myocardial ischemia reperfusion injury (MIRI). METHODS: Twentyª²four adult healthy crossª²bred canines were randomly assigned to four groups: Control group, ischemia preconditioning group, QDTMT low dosage group (QDTMTL) and QDTMT high dosage group (QDTMTH). Canines in all groups took QDTMT (or NS) orally for a week under the same condition. The model of MIRI was established by ligating levoª²anterior descending branch of coronary artery. The contents of ET, NO, TXB2 and 6ª²ketoª²PGF1¦Á in plasma were measured at four time points: Preª²drug (T1), 20 min after ligation (T2), 10 min (T3) and 30 min (T4) after reperfusion respectively and the equilibrium between NO / ET and that between 6ª²ketoª²PGF1¦Á/ TXB2 were analyzed. RESULTS: Compared with the control group, QDTMTL and QDTMTH reduced the level of ET and TXB2, promoted the expression level of NO, increased the activity of 6ª²ketoª²PGF1¦Á, and regulated the equilibrium between NO and ET and that between 6ª²ketoª²PGF1¦Á and TXB2 (P<0.05, P<0.01). There is no significant difference between QDTMT groups and the ischemia preconditioning group(P>0.05). CONCLUSION: Just as ischemia preconditioning, QDTMT has good protective effects on MIRI. The mechanism of protection relates to its function of decreasing the level of ET and TXB2, promoting the expression level of No, increasing the activity of 6ª²ketoª²PGF1¦Á, and regulating the equilibrium between NO and ET and the equilibrium between 6ª²ketoª²PGF1¦Á and TXB2. ......