冰片_____冰片；高效液相色谱法；衍生化;

高效液相色谱衍生化法测定脑脉康中冰片含量

http://www.100md.com 《第四军医大学学报》 2003年第5期

冰片,,冰片；高效液相色谱法；衍生化;,3,5二硝基苯甲酸冰片酯,0引言,1材料和方法,2结果,3讨论,【参考文献】

     HPLC determination of borneol in traditional Chinese medicine by derivatization

    SHI Ru, GAO SuLi

    Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xian 710033, China

    【Abstract】 AIM: To develop a new method for determining the borneol content in traditional Chinese medicine. METHODS: By precolumn derivatization with 3, 5dinitrobenzoyl chloride(DNBCl, approximately 20 times the mole ratio of borneol) catalyzed by 4dimethylaminopyridine(DMAP, approximately 40 times the mole ratio of borneol) in acetonitrile at room temperature，the reaction mixtures were injected into and separated on an ODS column with methanol∶water(88∶12) as mobile phase, and detected at 225 nm. RESULTS: The calibration curve was linear in the range of 1 ~ 10 nmol for borneol. The borneol contents of two batches of LaoMaiKang were 7.9% and 7.1% and RSD was 1.0% and 1.5% (n=5), respectively. The average recovery rate was 99.9% and RSD is 1.8% (n=4). CONCLUSION: HPLC derivatization method is simple and accurate for the determination of borneol content in traditional Chinese medicine. ......

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