关键词: 肿瘤坏死因子;脱噬作用;皮肤/损伤;内皮，血管

    摘 要:目的 探讨TNFα 引起的血管内皮细胞(VEC)凋亡在皮肤撕脱伤早期血栓形成中的作用. 方法 分2组，实验组:内皮细胞(EC)+TNFα ，拮抗组:用EC+TNFα +TNFα 抗体处理.采用流式细胞仪分别测定实验组TNFα 不同时间、不同浓度以及拮抗组EC凋亡率. 结果 EC凋亡率随TNFα 作用时间增加，0～48h EC凋亡率为(2.6±0.42)%～(40.5±8.3)%;EC凋亡率也随TNFα 浓度而增加，0～3000u mL-1 EC凋亡率分别为(2.6±0.42)%～(13.7±2.6)%;TNFα 抗体明显拮抗TNFα 诱导的EC凋亡(P<0.01). 结论 TNFα 可引起EC的凋亡和死亡，具有时间依赖性和浓度依赖性，人重组TNFα 单抗可消除因TNFα 引起的毒性作用.

    Tumor necrosis factor induces apoptosis in normal vascular endothelial cells in vitro

    LI Xing-Dong，LU Kai-Hua，GUO Shu-Zhong，GAO Zheng-Hui

    Department of Plastic Surgery，Xijing Hospital，Fourth Military Medical University，Xi'an710033，China

    Keywords:tumor necrosis factor;apoptosis;skin/injuries;endothelium，vascular

    Abstract:AIM To study the effect of TNFα on apoptosis of vascular endothelial cells(VEC)in early second thrombosis after skin avulsion.METHODS It was divided into experi-mental group(EC+TNFα )and inhibitory group(rhTNFα Ab pretreatment of EC+TNFα ).According to various times and concentration ......